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The following message was posted to: PharmPK
Dear all,
I really appreciate if anyone can suggest me the separation and
quantitaion techniques of enantiomers using LC-MS/MS for
Bioequivalence Study.
As per some of the referances I came to know that HPLC is more
suitable than LC-MS/MS but I am not sure. Any expert opinions on this
topic are invited.
Any suitable references or presentations previously presented at
conferencess are welcome.
Regards,
Santosh Tata
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The following message was posted to: PharmPK
Santosh,
Interesting question...in my experience, though, the prospect of doing
enantiomeric analyses for PK purposes has much *less* to do with the
method of detection (e.g., LC/UV vs. LC/MS) and *more* to do with some
early and additional attention to chromatography.
I have been very successful in using a variety of columns - from Human
Serum Albumin (HSA) columns to traditional "Pirkle" columns - to achieve
good separation and excellent detection limits (1 ng/mL by LC/MS) for
enantiomeric pairs of small molecules and their chiral metabolites as
well.
Best Regards,
Jim Schmidt
Sr. Scientist, Drug Metabolism and Pharmacokinetics
Lexicon Pharmaceuticals Inc.
8800 Technology Forest Place
DMPK B4 C222b
The Woodlands, TX 77381
jschmidt.-at-.lexpharma.com
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Dear Santosh
You can do it using the LC/MS/MS provided what you want. There is no
doubt that LC/MS is going to be much more specific and sensitive than
HPLC with traditional detectors. Regarding the separation there are
plenty of columns available which work pretty well with LC/MS specific
mobile phases. There are many vendors who sell these columns. Once you
achieve the separation its nothing different than analyzing two
analytes in one run.
Following are the links for the vendors for getting the chiral columns:
http://www.chiraltech.com/default.asp
http://www.chromtech.se/
http://www.esslab.com/daicel_chiral_columns.htm
Hope it will be helpful.
Thanks
Lakshmikant bajpai
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