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Dear All
Can any one suggest the pKa of Vitamin D3 (Cholecalciferol)? Also,
extraction procedure for the same without any derivatives. I am trying
to develop LCMS method.
Thanks
Bhavin Shah
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The following message was posted to: PharmPK
Dear Bhavin Shah
Regarding the second part of your question, extraction of Vitamin D3,
I could mention the following paper as a simple one:
Aksnes, L. (1992) A simplified HPLC method for determination of
vitamin D3, 25(OH)D2 and 25(OH)D3 in human serum. Scan J. Clin Lab
Invest 52: 177-182.
I hope it helps you.
Regards
Dr A. Rassouli
Dept Pharmacology
Faculty Vet. Med. University of Tehran
Tehran, Iran
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