Back to the Top
The following message was posted to: PharmPK
Can anyone recommend good conditions for detection of mannitol by
LCMS? I can detect the compound mass by direct infusion just fine,
but my chromatography is proving quite problematic. I have a
phenomenex Luna NH2 column (150 X 2 mm, 5 u). We have a Shimadzu 2010
HPLC connected to a ABI 3200 Qtrap. Any information about appropriate
buffers, flow conditions, gradient, etc would be greatly appreciated.
If there is another widely accepted compound that could be used to
monitor integrity of Caco-2 monolayers besides mannitol, that would be
greatly appreciated as well. I'm trying to avoid the use of
radioactivity, but if I can't work out the LCMS conditions, I may have
to resort to this. When one uses radioactive mannitol (and
propanolol) as a control, does it have to be added with your compound
of interest or can it be added later after you have completed your
uptake studies for your compound? How accepted is lucifer yellow?
Again, can that be added after compound studies are completed?
Want to post a follow-up message on this topic?
If this link does not work with your browser send a follow-up message to PharmPK@boomer.org with "Mannitol HPLC protocol" as the subject
Support PharmPK by using the
Copyright 1995-2011 David W. A. Bourne (email@example.com)