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Hi All, Just wondering if anyone here has experience working with
Neurotransmitters like glutamate, Acetylcholine, choline, serotonin,
GABA, histamine, myoinositol, 5HIAA etc? I worked with them before but
probably I was quite inexperienced in LCMS back then, I was quite
discouraged when I was trying to set up a separation method. I hope to
see if anyone here has experience to share on this. I was thinking of
separating them using API but was wondering if GABA needs to be
ionised with APCI since I read somewhere that it's better ionised on
APCI. Probably thinking of trying on C18 column but I read some of
them works better with ion-exchange. So I wondered how can I separate
all of them using a common separation method? Is it possible?
Nevertheless, I still need to try. Also, earlier on I think they
seemed to have solubility issues as they seemed not dissolving well in
ethanol (some but not all) so probably thinking of using methanol as
solvent this time. I welcome any experience sharing or advice as I
hope to get more ideas before I get started. Thanks.
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