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The following message was posted to: PharmPK
Just a couple of questions that popped up in my mind when I read the
PEG400 in monkeys question (Couldn't open the link though. Anyone has
the reference or an abstract?):
Does PEG (after repeated dosing or extended continuous infusion) affect
regulation of CYP expression/activity? Anyone know about this in any
species (mice? Rats?)?
Also, does PEG400 interfere with protein determination (eg determination
of total protein content in liver microsomes)? Bradford? Lowry? Biuret?
PEG is known to interfere quite often in LC/MS analysis of compound
concentrations and especially when trying to determine metabolite
structures. Any general tips and tricks to get around this?
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I can share my knowledge about PEG400 interfering with LC-MS/MS
analysis. PEG do break down at different values of source and compound
dependent parameters to give daughter fragments at the desired level.
This obviously occurs when you have PEG in the sample but this does
not occur when you process your sample for analysis, the fact being
that you use different compositions of Organic/buffer mixtures as your
reconstitution solution. In routine practice I have never observed PEG
traces interfering with the quantitation of drugs or metabolites.
Hope this helps.
Santosh Tata, BS (Pharm. Sci),
Clinsys Clinical Research Ltd,
C-46, Sector-62, NOIDA,
Uttar Pradesh, India.
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