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For evaluating the biocompatibility of certain products on normal
human cells, what is the criteria for the cell-line selection?
For example, I wish to evaluate the toxicity of dental products on
oral epithelial cells. In that case, from the variety of cell-lines
available, is it necessary that the studies be conducted on normal,
non-cancerous cell-line? since many of these cells were originally
isolated from carcinogenic tissue.
Secondly, will there be differences in the results if studies are
conducted on fibroblast cells as opposed to epithelial cells?
The reason I am asking this question is that, the 'human gingival
epithelial cells' that I am looking for is not commercially available
but ATCC has "human gingival fibroblasts".
Can anyone help me with this query??
Thanks a lot!!
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The following message was posted to: PharmPK
Normal cells in the gastrointestinal tract are impossible or extremely
difficult to culture. Therefore, human gingival fibroblasts are the
next best thing for the mouth region. Lower down, eg, the colon, the
same difficulty applies, so that abnormal cell lines such as Caco-2,
HT-29 and HCT116 cells are used. Therefore any findings using the cell
lines must be acknowledged as approximate indicators of responses and
any findings, positive or negative, must be validated in whole animal
or, preferably, human volunteer studies.
Des Williams, PhD, BPharm, FRACI
School of Pharmacy and Medical Sciences
University of South Australia
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