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I am having a question regarding estimation of fraction absorbed using in situ closed loop model.
As in this model amount transfered through gut loop changes with the time, so how to decide incubation time of our drug in intestinal gut loop.
we screened one of our standard compound in two different time 15min and 30min and got two different fraction absorbed of the same compound.I searched some literature but I did not find any time correction factor in fraction absorbed. I used the following equation for faction absorbed...........
Fraction absorbed=1-(Remaining GutVolume*Remaining Gut Concentration)/(Initial GutVolume*Initial Gut Concentration)
waiting for your expert comment.
[Buffer? Did you consider water transfer? - db]
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You can find out some answers to your questions in the following papers:
1. Terziivanov, D., I. Havlik and I. Janku. Evidence for saturable component in isoniazid transfer across rat small intestine in vitro. J Pharm Pharmacol.,1982; 34: 817, and
2. Farghali, H. et al. Biochem Pharmacol., 1984; 33: 655
Some methodological issues of in vitro drug transport are also summarized.
Dimiter Terziivanov, MD,PhD,DSc, Professor,
Univ Hosp "St. Ivan Rilski", 15 Acad. Ivan Geshov st, 1431 Sofia, Bulgaria
[The early work with this method or similar was at UK by Doluisio and Dittert, Diamond et al., various papers in J Pharm Sci. Thus called the Kentucky method or the Doluisio method ;-) - db]
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