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Is there any special technique for conducting Intravenous infusion
studies in mice?
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For continuous infusion in rodents, the jugular vein cannulation is
required. The major disadvantage of these technique is that the mouse
must be attached to a tether or swivel during the infusion period,
thereby restricting animal movement, preventing group housing of
animals, and adding cost and complexity to the procedure (Generally in
mouse PK study sparse sampling is involved so number of tether, swivel
and cages required will be more).
In past I tried the cannulation of jugular vein in Swiss Albino mouse
with PE-10 (I.D. 0.28 mm X 0.61mm O.D) and I got reasonably good
patency (~80 %).
To avoid the external connections and allowing the animals to remain
untethered and unrestrained during the entire infusion period, you can
go for ALZET(R) Osmotic pump (in this case also you have to cannulate
the jugular vein). You can also procure the small size cannula which
will make easy cannulation in mouse. The details are as below:
Description Item No. Length Outside Diameter Inside Diameter
(10 per bag) 0007760 15cm(6 in) 1.14 mm(0.045 in) 0.69 mm(0.027 in)
(10 per bag) 0007750 15cm(6 in) 1.22 mm(0.048 in) 0.76 mm(0.030 in)
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