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I am having problem regarding measurement of urea in hepatocytes as
cell viability marker. Wheather it is needed to lyse the cells or it
can be done from the supernatant of the cell culture medium, its a bit
confusing to me from the literatures. As also the medium composition,
as some have used ammoniam chloride, ornithin, as also lactate and
oleate, is not very clear. If somebody can share his/her experience on
this matter, it will be of great help.
Anticipating your co operation.
Katholieke University of Leuven
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