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We're developing a ELISA method for detection of the anti-drug antibody in cyno. monkeys, the drug is a human cytokine. We want to coat the drug in the plate, use goat anti-monkey IgG as detection antibody. How about the positive control? Just use a mice anti-drug monoclonal antibody? or monkey anti-body which we did not have yet? BTW, is anybody can give me some suggestion of the cut-off point setting, like the previously recommendation (Journal of Pharmaceutical and Biomedical Analysis 48 (2008) 1267-1281 ), a fixed cut-off point from 20 negative samples or just simple titers compared with the negative samples by serially diluted.
Thanks a lot
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