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I will run a study in rats, which is requested 1.6 mL/kg dose volume injected in one site. It is out of the recommended range for IM dose (0.1-0.2 mL per site). I would like to know if anybody have the experience with such large dose volume in rats? Or may anyone suggest a scientific publication on overview of the effects of the large dose volume on animals.
Any information would be much appreciated.
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In book titled " Anesthetic toxicity" by Susan A.Rice, Kevin J.Fish, the dosage volume guidelines recommendation in rat is given as follows (Page 29)
Ideal volume : 0.2 ml/kg
Limit volume : 2.0 ml/kg
[above, all one line - db]
In a research article titled " Dose-Dependent Amelioration of Gentamicin-Induced Nephrotoxicity in Adult Swiss Albino Rats by Vitamin B-complex -A Preliminary Study " published in Tropical Journal of Pharmaceutical Research, Vitamin B complex was given intramusculary at the dose of 1.5 ml/kg to Adult, female, Swiss albino rats, weighing170 +/- 20g.
Bello SO and Chika A, Dose-Dependent Amelioration of Gentamicin-Induced Nephrotoxicity in Adult Swiss Albino Rats by Vitamin B-complex -A Preliminary Study. Tropical Journal of Pharmaceutical Research, Vol. 8, No. 2, June 2009, pp. 111-116.
Overview of effects on large volume dose administration in animals:
Administration of excessive dose volumes may produce pain, excitement, altered physiological parameters (e.g., serum electrolyte imbalance, increased blood pressure, increased respiratory rate, etc.), and cause abnormal compound absorption...
Hull, R.M., Guideline limit volumes for dosing animals in the preclinical stage of safety evaluation, Human and Exptl Tox, 14, 305, 1995.
(Taken from "The Care and Feeding of an IACUC", Edited by: M. Lawrence Podolsky, M.D., Victor S. Lukas, D.V.M., pgs. 187 - 188.)
Thanks & Regards,
Clinical Pharmacologist & Principal Investigator,
Azidus Research Laboratories, Chennai.
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