PharmPK Discussion - Drug levels in ELF from BAL

• On 4 Aug 2010 at 08:07:13, David Benziger (David.Benziger.at.cubist.com) sent the message
  

  
  Does anyone have experience regarding method development and assay  validation for drugs (mostly antibiotics) in ELF?
  
  Any guidance appreciated.
  
  Dave
  
  

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• On 4 Aug 2010 at 15:52:24, Henri Merdjan (henri.aaa.novexel.com) sent the message
  

  
  David,
  
  As a starting point, let me recommend the following reading: Kiem and  Schentag, Interpretation of antibiotic concentration ratios measured in  epithelial lining fluid. Antimicrob. Ag. Chemother. 2008;52(1):24-336. I  believe it provides answers to most methodological aspects.
  Best of success!
  
  Henri
  
  Henri Merdjan
  Vice-President, Drug Metabolism and Pharmacokinetics
  Novexel, France
  
  

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• On 5 Aug 2010 at 09:34:34, Jitendar Reddy (sjeetu2u.-a-.gmail.com) sent the message
  

  
  Hi David,
   Paper mentioned by Henri Merdjan (VP - Novexel, France) is a review  article about different classes of antibiotics penetration into the ELF  and cellular components of the Lung
   Please find the below mentioned paper by A.Louie and G. Drusano from  Ordway Research Institute _ USA, that clearly explains the method  development and validation of the ELF collection by Bronco alveolar  Lavage (BAL) and its analysis.
  
  Pharmacodynamics of Levofloxacin in a Murine Pneumonia Model of  Pseudomonas aeruginosa Infection: Determination of Epithelial Lining  Fluid Targets, Antimicrob Agents Chemother. 2009 August; 53(8):  3325-3330
   Wishing you all the Best.
   Cheers,
  Jitendar Reddy
  AstraZeneca, Bangalore, India
  
  

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