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Hello Sir,
I was wondering which is a better anticoagulant: EDTA or heparin to be used in PK studies, as in which interferes less with the plasma and drug when being analysed by HPLC?
Thanks & regards,
Navdeep Kaur
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Hi Navdeep,
This depends on anticoagulant used for the Calibration method of HPLC. Usually EDTA is used.
Regards
Md. Tarique Imam
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Hello Sir
In addition to the previous question, I have a specific question too...
If I intend to use EDTA vacutainers can I use heparin flush to keep my cannula or catheter patent. Would there be any sort of interaction if I use this combination, although I plan to discard the initial few mls of blood containing heparin while taking multiple samples for PK study. Kindly suggest.
I want to use EDTA vacutainers as I read that EDTA interferes less in HPLC analysis as compared to heparin.
Thanks and regards,
Navdeep Kaur
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Navdeep,
There is no a rule of thumb as an answer to your question. My understanding is that in your particular case there is no a 'better anticoagulant' but an anticoagulant not interfering with the drug molecule you are intending to analyze for PK purposes.
Your question gets meaning in clinical setting when physician has to decide which is the most safe and therapeutically efficient anticoagulant for a particular patient, e.g., (standard) heparin or low molecular weight heparins.
In your case I recommend you a two steps approach. As a Step #1: To perform a set of pilot bioassays with both anticoagulants spiked with the drug molecule you are interested in PK analysis and to exclude mutual interference. As a Step #2: After choosing the appropriate anticoagulant you have to perform a set of bioassays to explore HPLC behaviour of the drug molecule in blood spiked with this anticoagulant and the drug of interest itself.
Regards,
Prof. Dimiter Terziivanov
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If possible, use EDTA especially if determining protein binding. Stanley Cotler
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Depends on what your drug is. Some drugs bind to heparin, and EDTA may inhibit/interfere with some analytical procedures.
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Dear Kaur,
try them as total recovery test with your drug and IS, if any.
All the best
Fabio
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The following message was posted to: PharmPK
Navdeep
In my experience EDTA works better than Heparin in most cases. Though there
appears to be a scarcity for K3EDTA vaccutainers in the US hence CRO's use
K2EDTA most frequently. Heparin works too but I cannot say for sure if it
would with your analyte. You could try out a preliminary experiment with
both anticoagulants.
Good Luck
Manish
Manish Issar, Ph.D
Project Manager
Applied Biopharmaceutics, LLC
2010 Cascade Drive
Corona, CA 92879
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Hi Navdeep
I prefer use lithium heparin because EDTA traps the divalent cations and this can modify the ionization of plasma, some buffer and EDTA could interfering with some drug molecules (salts).
Francoise BREE
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Dear Kaur
This also depends on the chemical nature of the drug if drug chelate then I will go heparin otherwise EDTA is good for HPLC assay.
zafar_iqbal.-at-.upesh.edu.pk
--
Navdeep,
Think about metabolite(s), if any, of this drug molecule and its(their) HPLC behaviour along with the parent drug.
Regards,
Prof. Dimiter Terziivanov
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Hi Navdeep,
There will be no problem because you are discarding heparinised blood.
All Tha Best
Best Regards
Md. Tarique Imam
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