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Dear friends,
We are developing a method for Glucosamine, but we are unable to get the expected recovery.
We have undergone all the extraction techniques still we are strugling to get the same.
It will be very fruit-ful, if you suggest some methodolgy for estimating Glucosamine from human plasma.
Thanks and regards,
MUTHU SWAMY.C,
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Have you looked on pubmed, column manufacturers sites, or the j clinical chemistry site?
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Hi Muthu,
Can you shed some light on various the extraction methods you followed.
Cheers
Ravi
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Glucosoamine is a water soluble sugar compound and LLE is not preferred.
Did you try protein precipitation? Also, you may need to use an amino
column. IF you are trying to use C18 column, you can consider adding a
suitable ionpair chromatography.
Regards,
Vinayak Nadiger
Vinayak Nadiger
Manager, Bioanalytical
Forma Therapeutics(Singapore)
11,Biopolis Way ,Helios # 08-05
Singapore 1386607
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Dear Sir,
Can you kindly explain about ion pair chromatography, is that required to add ion pairing agent? kindly suggest some ion pairing agent if it is required to add.
hope your sugestion will help us to reach our goal.
Thanks and regards,
MUTHU SWAMY.C,
TEAM LEADER,
BIOANALYTICAL DEPT, QUEST LIFE SCIENCES PVT.LTD.
SDF-III, MEPZ
TAMBARAM, CHENNAI- 600 045
TAMIL NADU, INDIA
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If you are using C18 column, basic ion pairing agents need to be added.
You may need to experiment with various ion pairing agents available. To
start with try one of amines available in your lab-like Cyclohexyl
amine, cycloheptyl amine etc. Triethyl is more popular, but I have seen
signal suppression in mass spec with this ionpairing agent. If you need
more guidance, you can discuss with me offline.
Regards,
Vinayak Nadiger
Vinayak Nadiger
Manager, Bioanalytical
Forma Therapeutics(Singapore)
11,Biopolis Way ,Helios # 08-05
Singapore 1386607
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Dear Muthu Swami,
I use the ion pair chromatography for quantification of endogenous basic compounds in tissue and biological fluids. My ion pair agent is octanesulphonate sodium salt. This molecule bind the protonate group of my compound to form the "ion pair". The "ion pair" has a specific partition coefficient and with regard to partition coefficient the "ion pair" distribute among the stationary phase or mobile phase.
I clarify that my sample are acidic medium that is indispensable for tissue and biological fluid extraction of my compounds. My method is "ion pair" reverse phase chromatography.
I hope to give you some useful indications.
Best regards.
Raffaella Bombelli
Section of Experimental and Clinical Pharmacology
Department of Clinical Medicine
University of Insubria
Varese, Italia
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