PharmPK Discussion - Acamprosate calcium lc-ms-ms method

• On 21 Jul 2011 at 18:23:53, raghuram (raghuram.-a-.bioserve.co.in) sent the message
  

  
  Dear all,
  
  As we are developing method for quantification of Acamprosate in human plasma we are not
  at all getting  recovery for the analyte and facing lot of problems to reduce
  
  Endogenous interference in matrix Samples.
  
  pls suggest us to get a good bioanalytical method regarding this.
  
  Presently we used Ammonium acetate pH 4.5 and methanol (40:60) and also Hypersil BDS C18
  column 150*4.6 5um column.
  
  We are not getting enough recovery with protein precipitation performed with MeOH,MeOH
  with 1% Formic Acid.
  
  We have also tried with SPE and LLE with different solvents and Sobents beds.
  
  Awaiting for ur positive Response
  
  Thanks and Regards,
  
  A.V.Raghuram,
  

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• On 24 Jul 2011 at 22:12:34, "NADIGER, Vinayak" (vnadiger.at.formatherapeutics.com) sent the message
  

  
  The following message was posted to: PharmPK
  
  Hi Raghuram,
  Did you check whether Acamprosate is retaining well on this column?
  Looking at the structure, I guess, it is eluting at void, where matrix
  components are also coeluting. Try using alternate column chemistry, if
  it helps.
  
  Regards,
  Vinayak Nadiger
  
  Vinayak Nadiger
  Senior Scientist
  Forma Therapeutics(Singapore)
  11,Biopolis Way ,Helios # 08-05
  Singapore 1386607
  
  

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• On 28 Oct 2011 at 17:00:00, raghu ram (avrmscorg.at.yahoo.co.in) sent the message
  

  
  Hi All,
  Can anybody suggest us a good bioanalytical method for Acamprosate as
  the Acamprosate was co-eluting along with phospholipids.We have used
  Atlantis HILIC column as stationary phase and Sample preparation was
  done in Oasis MCX and also in WAX.But no recoveries were Observed in the
  Column.
  
  Thanks and Regards
  A.V.Raghuram
  
  

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• On 31 Oct 2011 at 07:17:29, Xiaodong Shen (shenxiaodong11.-at-.yahoo.com) sent the message
  

  
  Hi,
  
  I may suggest not using Atlantis HILIC column. This column does have a
  polar retention function, but it also has an cation exchange mechanism.
  Acamprosate has a strong acidic group, you may want to try  a HILIC
  column with anion exchange mechanism.
  
  Hope this help.
  
  Xiaodong Shen
  
  

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