PharmPK Discussion - Calibration curve with DBS DMPK cards

• On 2 Feb 2011 at 10:58:09, (VijaykumarS.-at-.ilsresearch.org) sent the message
  

  
  Dear all
  We are planning to conduct an exposure study in zebra fish.   Coming to  sample collection, we planned to collect the samples onto DMPK cards  Since the sample volume could be less.
  My question is regarding the preparation of calibration curve.
  Usually we follow serial dilutions method to prepare the analyte CC  concentration in suitable medium like DMSO  in such a way the  concentrations are sufficient enough to get the ultimation CC  concentrations in the matrix (i.e, 1-2ul volume from analyte CC in to  the matrix not less than 100ul ). And the processing method would be any  of the conventional extraction methods.
  As the blood volume in zebra fish is very less, it is quiet challenging  to prepare the CC standards.
  Can any body suggest me the suitable procedure to prepare CC standards  in less volume of matrix.
  If iam not clear in any regard, please let me know.
  Regards
  Vijaykumar.S
  Senior Research Associate,
  COSMIC Discoveries,
  Institute of Life Sciences,
  University of Hyderabad Campus,
  Gachibowli, Hyderabad 500046
  
  

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• On 2 Feb 2011 at 13:10:30, "Edward O'Connor" (efoconnor.-a-.gmail.com) sent the message
  

  
  The following message was posted to: PharmPK
  
  Use a surrogate matrix- from a larger fish or artificially created,
  worry about matching matrices when you get into mammals.   Express
  result as relative to curves and QCs prepared in the surrogate
  matrix-be very clear about this.
  
  

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• On 4 Feb 2011 at 10:45:23, "Rajanikanta Sahu (5318)" (rajanikanta.s.at.saiadvantium.com) sent the message
  

  
  The following message was posted to: PharmPK
  
  Dear Vijaykumar
  
  Ideally you have to prepare CC in same matrix as of sample. I understood  your limitation is availability of matrix volume! To solve this problem  you can cross validate the extraction method with different matrix  (Blood from different species), evaluation of recovery will be enough to  cross validate the method with two different matrices (zebra fish. Vs  matrix X)
  If you can develop a method with similar recovery for both the matrix U  can use a Calibration curve in validated matrix and easily quantitate  your sample
  
  Hope this will solve your problem
  
  Rajanikanta Sahu
  Research Scientist-Bioanalytical (DMPKT)
  Saiadvantium Pharma Ltd.
  
  

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• On 8 Feb 2011 at 07:52:45, vijay sripuram (sripuramvijay.-at-.gmail.com) sent the message
  

  
  Dear Rajanikanth
  
  Thanks for your reply.The same idea was with me but in order to cross  validate the extraction method , do i need to go for all the CC points  or shall i go for limited number(since there is another limitation for  DMPK cards).
  
  

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