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We had analysed the Liposomal formulation of one drug in different tissues of Rat & mice. For the analysis of free and encapsulated form of the drug samples were processed immediately after sample collection. For the estimation of Total drug samples another aliquot of samples were store in deep freezer (-35*C) and was process later on.
So what we assume that Total drug = Free form + Encapsulated form
But to our surprise above theoretical equation was not establised in most of the Rat and mice tissue, There was more than 50% variation between the practical value of Total drug and value obtained from the above equation. Looking analytically all the CC and batch QC were within acceptacne for every accepted batch.
Can anybody suggest reason for such varation.
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