PharmPK Discussion - Flavin monooxygenase activity

• On 28 Mar 2011 at 15:17:40, vijaya bhargava (vijayabhargava.k.at.gmail.com) sent the message
  

  
  The following message was posted to: PharmPK
  
  Dear all,
  
  We have been trying for establishing Flavin monooxygenase (FMO)
  activity in our lab using Benzydamine as the standard substrate in
  liver microsomes, But even though we performed the assay using the
  described conditions in the literature, w e could not notice any
  metabolism of benzydamine. The pre- incubation of microsomes was done
  with NADPH before the reaction started with the addition of
  benzydamine at a final concentration of 2.5uM. Can anyone give me the
  reason of the observed no metabolism or say (20%) itself.
  
  Thanking you
  Vijayabhargava.k
  
  

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