PharmPK Discussion - Permeability using MDR1-MDCK cells

• On 20 Jul 2011 at 12:00:35, Mansi Sharma (mansish.aaa.glenmarkpharma.com) sent the message
  

  
  The following message was posted to: PharmPK
  
  While calculating efflux ratio in MDR1-MDCK cell based permeability screen, is it always
  necessary to compare  efflux ratio of transfected cell line to that of parent cell line
  (non transfected MDCK)?
  Could somebody please advise?
  
  Regards,
  Mansi Sharma
  

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• On 20 Jul 2011 at 14:27:50, (agar0077.-at-.umn.edu) sent the message
  

  
  The following message was posted to: PharmPK
  
  Hi Mansi,
  Comparing the Efflux ratio in the transfected cell line to the parental cell line gives an
  estimate of the true magnitude of P-gp mediated efflux. This is because there is native
  P-gp that may be present in the parental cell line and can contribute to drug efflux
  (MDCKII is a kidney cell line which can express some P-gp)
  
  Therefore we routinely calculate what is known as the corrected flux ratio (CFR) which is
  basically the ratio of efflux ratio in transfected to that in parental cell line. { CFR =
  Efflux ratio in transfected cell lines divided by Efflux ratio in parental cell line }
  
  Hope this helps,
  Sagar
  

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