PharmPK Discussion - Protein Binding

• On 23 Dec 2011 at 09:08:05, nagsen gautam (nagsengautam.aaa.gmail.com) sent the message
  

  
  The following message was posted to: PharmPK
  
  I'm characterizing the plasma  protein binding of a compound.  The
  compound has extremely low solubility i.e. spiking PBS or plasma
  filtrate with 100 ng/ml in plastic vial, the compound is completely
  gone.  To check nonspecific binding with filter, we try to check in
  PBS and Serum filtrate. We not able to recover compound in PBS or
  serum filtrate without filtration.  Apparently the compound is lost to
  precipitation, the vial walls, and filter binding.  Does anyone have
  experience with such type of compounds?  How will you account for such
  extremely high nonspecific binding? Any methods or products better
  than others?
  
  Thank you
  
  --
  Nagsen Gautam
  
  

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• On 23 Dec 2011 at 14:05:38, Stanley Cotler (msgcot2.-at-.optimum.net) sent the message
  

  
  The following message was posted to: PharmPK
  
  Look at Harvards 96 well plates, they seem almost immune to non-specific
  binding.
                                      Stanley Cotler
  
  

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• On 23 Dec 2011 at 17:45:36, P C (parnalic.at.hotmail.com) sent the message
  

  
  Please try adding 0.1-1% formic acid, EDTA, etc (titer to see what conc
  works and if your compd is stable in formic acid) to see if you can
  prevent non-specific binding. You can also try low bind PP tubes. All
  tubings should also be replaced with PEEK tubing to prevent any
  non-specific binding.
  
  Hope this helps.
  
  Parnali Chatterjee
  E-mail: Parnalic.at.hotmail.com
             parnali_chatterjee.at.yahoo.com
  
  

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• On 24 Dec 2011 at 08:39:28, satya jadhav (satyawanjadhav.-at-.gmail.com) sent the message
  

  
  You may want to try Rapid Equilibrium device (RED) or
  ultracentrifugation method.
  
  

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• On 24 Dec 2011 at 17:26:50, abhijith rao (abhijithrao88.at.gmail.com) sent the message
  

  
  0.1% formic acid should help ( in case the compd is stable in acidic
  conditions!)
  
  

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