PharmPK Discussion - Query on BCRP Transporters

• On 23 Nov 2011 at 09:57:11, Himanshu Rastogi (himanshu.r.aaa.saiadvantium.com) sent the message
  

  
  Dear Group Members
  
     I wanted to know whether BCRP transporter assay can be set up using
  Caco-2 cells.
  
  Regards,
  Himanshu
  
  

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• On 23 Nov 2011 at 13:15:58, (vinodpatil.at.lupinpharma.com) sent the message
  

  
  The following message was posted to: PharmPK
  
  Dear Himanshu,
  
  Yes, you can . you can use selective substrate as 3 Estron Sulfate and
  inhibitor as FTC
  
  Thanks & Best Regards
  
  Vinod Patil
  
  

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• On 23 Nov 2011 at 08:18:54, OSMAN-PONCHET Hanan (Hanan.OSMAN-PONCHET.-at-.galderma.com) sent the message
  

  
  We have measured the expression level of four well known ABC
  transporters in Caco-2 cells (BCRP, MRP1, MRP2 and P-gp or MDR1).
  All the four transporters are expressed in Caco-2 cells at mRNA level.
  Expression of MRP2 is the most important followed by MRP1 and MDR1
  (representing about 30% of MRP2).
  Expression of BCRP in Caco2 cells is however, very low (only 1% of
  MRP2).
  
  Kind regards,
  Hanan Osman-Ponchet
  Metabolism Manager
  Galderma R&D
  Hanan.osman-ponchet.aaa.galderma.com
  
  

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• On 23 Nov 2011 at 15:14:34, Masoud Jamei (masoud.jamei.-a-.gmail.com) sent the message
  

  
  Hi Himanshu
  
  You may find the following papers useful:
  
  http://dmd.aspetjournals.org/content/37/9/1956.abstract
  http://dmd.aspetjournals.org/content/early/2011/03/29/dmd.111.038075.
  abstract
  
  Regards
  Masoud
  
  

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• On 23 Nov 2011 at 20:51:59, "Xia, Cindy" (Cindy.Xia.at.MPI.com) sent the message
  

  
  The following message was posted to: PharmPK
  
  Attached please find our paper about characterizing BCRP protein
  expression and function in Caco-2 cells.
  
  http://dmd.aspetjournals.org/content/33/5/637.short
  
  Best Regards,
  
  Cindy
  
  

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