PharmPK Discussion - Screening of highly lipophilic compounds

• On 21 Jun 2011 at 09:21:22, Mansi Sharma (mansish.aaa.glenmarkpharma.com) sent the message
  

  
  The following message was posted to: PharmPK
  
  Hi, I got a small query on the screening of highly lipophilic compounds in  cell based assays. We are working with some highly potent compounds which are quite  insoluble even at lower concentrations of 0.1 uM in some of the usual  buffers used in microsomal and hepatocyte assays e.g. phosphate buffer,  Williams E media etc. We are using 0.5% DMSO concentration for cell based assays, but, the  moment these are being spiked in buffer, are getting precipitated. Could  anybody suggest if we could use bovine serum albumin or serum or  something else to keep these compounds solubilized in these buffer to  assess their metabolic pattern? I haven't come across any literature on  same. Any suggestions?
  
  Thanks,
  Mansi Sharma
  
  

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