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Hi, I got a small query on the screening of highly lipophilic compounds in cell based assays. We are working with some highly potent compounds which are quite insoluble even at lower concentrations of 0.1 uM in some of the usual buffers used in microsomal and hepatocyte assays e.g. phosphate buffer, Williams E media etc. We are using 0.5% DMSO concentration for cell based assays, but, the moment these are being spiked in buffer, are getting precipitated. Could anybody suggest if we could use bovine serum albumin or serum or something else to keep these compounds solubilized in these buffer to assess their metabolic pattern? I haven't come across any literature on same. Any suggestions?
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