PharmPK Discussion - Protein binding centrifuge speed

• On 11 Jan 2012 at 11:54:04, Lisa Black (liese82.-at-.gmail.com) sent the message
  

  
  Hi all,
  
  I am getting ready to do the protein binding assay for my pharmacokinetic study using
  ultrafiltration with 30kDa centrifugal filters
  http://www.millipore.com/catalogue/module/c82301#0
  
  The product information leaflet says to spin at 14,000 x g for 10-15 minutes, however the
  general protein binding method I have looked at says to spin at 1,000 x g for 20 minutes.
  Is there a reason they have recommended spinning at such a slow speed? Does anybody have
  any experience or recommendations for the filter I am planning to use, or for protein
  binding assays in general?
  
  Any advice would be appreciated!
  
  Thanks!
  
  - Lisa
  

  Back to the Top

• On 10 Jan 2012 at 21:23:32, Sanjeev Thohan (sarxconsult.at.gmail.com) sent the message
  

  
  Dear Lisa, look at the conversion tables for the centrifuge that you are using and
  calculate the adequate g based on the centrifugal radius. The issue with some of the
  filters is that too much g for too long will cause a physical failure of the filer, too
  little and you will not get accurate readings.
  
  sanjeev
  

  Back to the Top

• On 11 Jan 2012 at 13:27:01, wangchangguang (chg-wang.-a-.hotmail.com) sent the message
  

  
  I think you can change the speed, the only thing you should thought about is that the
  ultrafiltration should no more than 20% of the original plasma volume.
  thanks
  

  Back to the Top

Copyright 1995-2011 David W. A. Bourne (david@boomer.org)