PharmPK Discussion - Repeated IP Injections to Mice

• On 24 Jan 2012 at 16:30:43, Alene McCoy (alenemccoy.at.gmail.com) sent the message
  

  
  The following message was posted to: PharmPK
  
  Hello group,
  
  I was just wondering if anyone would have any references regarding
  repeated IP injections in a single day in mice. Specifically, what is
  the maximum number of IP injections per day that is recommended?  Does
  anyone know of any guidance on this?
  
  Thanks,
  
  Alene
  
  

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• On 25 Jan 2012 at 18:39:48, Sanjeev Thohan (sarxconsult.-at-.gmail.com) sent the message
  

  
  Here is one reference you may find helpful with regards to IP injection volumes. One must
  also consider the composition of the injection and its osmotic effects on blood volume in
  the mouse if you are determining PK and exposure.:
  The Journal of Infectious Diseases  Vol. 130, No. 2, Aug., 1974  page 209
  http://www.jstor.org/stable/i30106986
  
  Here is another reference:
  
  weight (g) and volume to be administered in 1 injection(mL)
  
  Mouse <10   0.25
       10-15  0.5
       15-20  1.0
         >20  1.5
  
  

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• On 26 Jan 2012 at 09:27:38, Alene McCoy (alenemccoy.aaa.gmail.com) sent the message
  

  
  The following message was posted to: PharmPK
  
  Hi Sanjeev,
  
  Thank you for your reply.  I was actually more wondering about number
  of injections, rather than injection volume.  I'm trying to find some
  guidance on how many IP injection can be done in a day without causing
  excessive stress to the mice.
  
  Thanks,
  
  Alene
  
  

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• On 26 Jan 2012 at 18:25:02, "Beaupre,AJ" (AJ.Beaupre.aaa.colostate.edu) sent the message
  

  
  The following message was posted to: PharmPK
  
  Alene,
  
  Unfortunately I don't have a reference, but I've done 4 to 6 injections
  within a 24 h period without much difficulty.  Any more than that and
  you may want to consider an osmotic pump implant.
  
  -AJ
  
  

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• On 26 Jan 2012 at 21:55:31, "Ed P'Connor" (efoconnor.at.gmail.com) sent the message
  

  
  The following message was posted to: PharmPK
  
  I worked with an ip catheter in mice and rats with that it was unlimited!
  
  

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• On 26 Jan 2012 at 22:56:27, Sanjeev Thohan (sarxconsult.aaa.gmail.com) sent the message
  

  
  IACUC guidelines for the institution that you are working with with usually will have
  guidances, but 4 is about as many as I would go in an alternating left and right manner.
  Also keeping the needle to a 28g butterfly works better.
  
  Sanjeev
  

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