PharmPK Discussion - Tetrabenazine Method Development

• On 17 Mar 2015 at 11:39:35, Mythies Kumar Sellamuthu (mythies2001in.-at-.gmail.com) sent the message
  

  
  Presently I am working on Method development of Tetrabenazine and its active metabolite, HTBZ. As
  per OGD guidance, the analyte to be measured is Tetrabenazine and its active metabolite, HTBZ in
  plasma using achiral assay. HTBZ (Tetra hydrobenazine) is in alpha and beta forms, I need to know
  whether to use the racemic standard of HTBZ or to use alpha and beta standards separately for
  analysis since its an achiral assay.
  
  Thank you
  
  S.Mythies Kumar
  
  

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• On 17 Mar 2015 at 12:04:45, naveed shaik Abdul (naveedshaik.-at-.gmail.com) sent the message
  

  
  Hello Kumar,
  
  As the plasma samples will be obtained after a dose of racemic tetrabenazine you should be measuring
  the racemic tetrabenazine and HTBZ  using a "achiral method" rather than chiral method. If the dose
  is individual enantiomer than you will be using a chiral method with pure enantiomers i.e. in your
  case alpha and beta standards.
  
  I hope this clarifies.
  
  Regards
  
  Naveed
  
  

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