PharmPK Discussion - Erythrocyte binding Kd and Bmax determination with scatchard plot

• On 26 Jul 2016 at 10:24:58, (R.terHeine.aaa.radboudumc.nl) sent the message
  

  
  Dear fellow pharmpk readers,
  
  I am trying to determine the erythrocyte binding constant (kd) and bmax of a drug, based on
  information in the FDA clinical pharmacology and biopharmaceutics review. I have extracted
  quantitative data from a graph showing relative distribution of a drug in erythrocytes and plasma
  versus whole blood concentrations.
  
  For contruction of a scatchard plot, I plot the bound:free ratio on the Y-axis, but as the X-axis
  should contain the specific binding, I am confused what I should use here. Should I put the whole
  blood concentration there, or should I correct for hematocrit (which is unknown)?
  
  Looking forward to your suggestions!
  
  Rob
  
  

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• On 0 0 at Date , (R.terHeine.-at-.radboudumc.nl) sent the message
  

  
  Dear fellow pharmpk readers,
  
      I am trying to determine the erythrocyte binding constant (kd) and bmax of a drug, based on
  information in the FDA clinical pharmacology and biopharmaceutics review. I have extracted
  quantitative data from a graph showing relative distribution of a drug in erythrocytes and plasma
  versus whole blood concentrations.
  
      For contruction of a scatchard plot, I plot the bound:free ratio on the Y-axis , but as the
  X-axis should contain the specific binding, I am confused what I should use here. Should I put the
  whole blood concentration there, or should I correct for hematocrit (which is unknown)?
  
  Looking forward to your suggestions!
  
  Rob
  
  

  Back to the Top

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