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I am interested in hearing about your experiences with tissue
homogenizers - especially the pros and cons of Potter-Elvehjem (teflon
coated rods?) versus the handheld electric "tissue grinders". The goal is
to prepare liver microsomes. Thank you in advance.
J. Vora
vora.-at-.butler.edu
Butler University
Indianapolis, IN, USA
from: VORA 940-9216
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It has been my experience that the PE tissue homogenizers work very well
for liver. Liver tends to be a soft tissue ang grinds easily. Other
tissues, for example gut tissues, tend to get wrapped up in the grinder
and may jam it, thus breaking the homogenizer ( and they are expensive to
replace). The problem that we have encountered with the electric
homogenizers for microsomal work is that they tend to generate alot of
heat and may inactivate microsomes. Even the PE tends to generate some
heat, and we place the grinder in an ice bath while we grind. The other
problem with the electric homogenizers is that it is sometimes difficult
to get all the tissue back out of them without excessive rinsing. It is
easier to rinse the PE homogenizer.
Hope that wasn't to graphic for anyone !!
Brian Corrigan
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