PharmPK Discussion - In Vitro to In Vivo scaling for metabolic parameters

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On 22 Sep 1997 at 10:20:00, A J Macdonald (MDP95AJM.aaa.shef.ac.uk) sent the message


Please can you post this to the newsgroup. Thanks

I am developing some human PBPK models for some volatile organic
compounds.I have Km and Vmax data based on in vitro human liver
microsomes.When scaling to in vivo I need a value for mg of microsome per
gram of liver. I have been using a range of 10-50mg per g. Can anyone
offer a better estimate or point me in the direction of some literature?
I have found a couple of papers for rat data but none for humans.

Regards
Alex MacDonald
Graduate Student
Dept of Medicine and Pharmacology
University of Sheffield
UK

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On 23 Sep 1997 at 10:48:32, Chandrani Gunaratna (prema.aaa.bioanalytical.com) sent the message


Dear Alex:

A good article on in vivo-in vitro correlation for human liver is

"Prediction of in vivo drug metabolism in the human liver from in vitro
metabolism data", Takafumi Iwatsubo et.al. Pharmacol. Ther. vol 73, no. 2,
1997 147-171

Hope this helps.

Chandrani Gunaratna

Chandrani Gunaratna, Ph.D.
Senior Research Chemist
Bioanalytical Systems
2701 Kent Avenue
West Lafayette, IN 47906
Phone: (765)463-4527
E-Mail: prema.aaa.bioanalytical.com

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