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In our lab, we have done some work on N-oxide drug metabolites. I believe,
with respect to polarity, N-oxides and S-oxides are quite similar (V.V.
polar). As you
also noticed, with these compounds reverse phase extraction (and may be
even reverse phase HPLC due to lack of sufficient retention) may not be
very successful. With our compound, an amino phase operated in
"pseudo normal phase" mode (starting with acetonitrile and gradually
increasing water) provided excellent retention on the HPLC column. You may
want to try this phase for solid phase sample extraction. Good luck and
please do let me know if it works.
On Wed, 8 Jan 1997, SHASHI KUMAR VET TOXICOLOGY wrote:
> PharmPK - Discussions about Pharmacokinetics
> Pharmacodynamics and related topics
> I am working on the compound Thioacetamide and its metabolites Thioacetamide
> suof oxide, and acetamide. I am quantitaing these from the blood and liver
> tissues by HPLC. These compounds are highly polar. I am employing
> liquid liquid extraction using ethylacetate as a solvent and found that I am
> not getting sufficient recovery. also looks like the procedure is not clean
> and reproducible. I am also presently trying reverse phase solid phase
> extractions. THese compounds have high solubility in ethanol followed by
> water. Thioacetamide was earlier used as an antifungal agent and is no more
> used pharmacologically.
> I would want some input with regard to extractions from these biological
> fluids for quantitation.
> If you need any more information on these, i can provide for you,
> looking for a reply,
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