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I've been assaying tobramycin in sputum (made up of DNA and mucin) using
an HPLC techniques which involves derivatization with phthaldialdehyde
followed by fluorescence detection.
I first hydrolyse the sputum in sodium hydroxide which has the effect of
solubilising all the "tissue" components. I then derivatize and assay
If you'd like to know more you can contact me at
Darren A. Saunders
Clinical Pharmacology Dept
New Zealand :)
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