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Dear Pharmacokineticists
I want to get the CaCo2-2 cell line. Can somone recommend me how to get
it. THank you very much.
Sincerely yours
Asst. Prof. Dr. KOrbtham Sathirakul
Mahidol University
Bangkok, Thailand
Tel: 66-2-644-8694
Fax: 66-2-247-4696
e-mail:pyksk.aaa.mahidol.ac.th
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Attn: Cell Culture people:
I recently had this experience with Caco2 cells. They grow happily in
the transwells/snapwells for about 2-3 weeks but during the last few
days ( the 18-21st day) they start to come off the polycarbonate support
in the transwells while changing media.
I wish to know wheather its routine practice to coat the transwells with
collagen ? Is there any particular reason why these cells start to lose
the monolayer after weeks ?
How to troubleshoot this ? am trying collagen coating now. any other
suggestions ?
I would appreciate any inputs.
Thank you,
Pankaj
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