PharmPK Discussion - Flukonazole HPLC with fluorometric detection

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On 26 Aug 1999 at 13:00:01, Kazimierz Kozlowski (khkoz.-at-.czd.waw.pl) sent the message


From: Kazimierz H. Kozlowski, Pharm. D.
       Laboratory of Pharmacokinetics
       The Children's Memorial Health Institute
       04-736 Warsaw, Poland
       E-mail: khkoz.-at-.czd.waw.pl

I am looking for liquid chromatographic method for spectrofluorimetric
detector
for analysis of fluconazole in pediatric serum samples. In available
literature
exist HPLC-SF methods for ketoconazole or itraconazole, but in the case
fluconazole authors used the only UV detection. I am interesed in if
fluconazole possesses native fluorescence or not, if so in what conditions.
My project is
pharmacokinetic and pharmacodynamic drug interaction between antifungal and
cytostatic drugs in children with solid tumors (ifosfamide, cisplatin,
carboplatin and fluconazole, Amphotericin B + flucytosine)

						sincerely
                                           Kazimierz H. Kozlowski

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On 26 Aug 1999 at 21:17:28, Leslie Carstensen Floren (leslief.-at-.itsa.ucsf.edu) sent the message


We run fluconazole with UV detection since it is a very poor chromophore.
It does not have native fluoresence and would, therefore, need to be
derivitized.
Sincerely,
Leslie C. Floren, Pharm.D.
Assistant Professor Biopharmaceutical Sciences
UCSF School of Pharmacy

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