PharmPK Discussion - Ropivacaine assay method

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On 27 Oct 1999 at 23:41:26, "Marco Calamandrei" (mcalamandrei.aaa.mclink.it) sent the message


Dear All
We are trying to dose ropivacaine in blood using mass spectography (HP), but
our technicians meet a lot of problem.
Could anyone give me advices?
Would we change to less sensitive methods?

Marco Calamandrei, Department of anesthesia and Intensive care
"A.Meyer" Children's Hospital
  Firenze Italia

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On 28 Oct 1999 at 21:59:10, "Jean-Xavier.Mazoit.aaa.kb.u-psud.fr" (Jean-Xavier.Mazoit.-a-.kb.u-psud.fr) sent the message


All techniques used for assaying other LAs (bupivacaine,...) are perfect.
Liquid extraction followed by GC with a nitrogen specific detector is the
best with a limit of detection of about 10 ng/ml
HPLC after solid phase extraction and UV detection at 210 nm is also possibl=
e.
Anton Burm from Netherlands knows perfectly all these problems.

Jean Xavier Mazoit MD, PhD

Laboratoire d'Anesth=E9sie
Universit=E9 Paris-Sud
Facult=E9 de M=E9decine du Kremlin-Bic=EAtre
F-94276 Bic=EAtre France
Tel.	(33) (0)1 49 59 67 35-37
	(33) (0)1 45 21 34 41 (Hopital)
Fax	(33) (0)1 45 21 28 75
e-mail	Jean-Xavier.Mazoit.-a-.kb.u-psud.fr

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On 30 Oct 1999 at 10:57:17, "Geng, Wanping {DRUG~Nutley}" (WANPING.GENG.aaa.ROCHE.COM) sent the message


There is very clean HPLC assay for amide type LAs (bupivacaine and
mepivacaine).  Michaelis, H.C., W.P. Geng, G.F. Kahl, and H. Foth:
Sensitive determination of bupivacaine in human plasma by HPLC. J.
Chromatogr. 527:201-207, 1990. Good luck.

Wanping Geng, MD

Roche
Tel: (973) 235-3532

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