PharmPK Discussion - Bile duct cannulation in rats

PharmPK Discussion List Archive Index page

On 25 Apr 2000 at 21:38:49, Hans-Markus.Bender.at.merck.de sent the message


Dear Vishwanath!
In our lab we use a non sterile Polythene Tubing with an inner=20
diameter of 0.4mm
and an outer diameter of 0.80mm for rat bile duct cannulation. For example:
Portex (UK), REF 800/110/140. The tube is distributed by the Lab=20
division of our
company MERCK. The following adress may be helpful for you:

Merck (India) Ltd.
Shiv Sagar ?Estate? A
Dr. Annie Besant Road
P. O. B. 16554
Bombay 400018
Tel: ++91-22-4964855
=46ax: ++91-22-4950307
E-mail: Ipro.mktg..at.emil.sprintsmx.ems.vsnl.net.in

Best regards
Markus
--------
Dr. Hans Markus Bender
Merck KGaA
Institut f=FCr Pharmakokinetik und Metabolismus
Am Feld 32
D-85567 Grafing
Tel: 08092 / 7008-0
=46ax: 08092 / 7008-99
e-mail: hansmarkus.bender.aaa.merck.de

Back to the Top

On 27 Apr 2000 at 20:02:27, korbtham sathirakul (pyksk.-at-.mahidol.ac.th) sent the message


We are working on the preclinical PK studies for many compounds and doing
the bile duct canulation routinely. We normally use the PE10 tube for the
canulation.
Best regards
Assoc. Prof. Dr. Korbtham Sathirakul
Faculty of Pharmacy, Faculty of pharmacy
Mahidol University, Bangkok, Thailand
and
Pharmcokineticist postdoctorate at Lilly-National University of Singapore
Singpore

Back to the Top

On 10 Nov 2000 at 09:53:39, Candice Kissinger (candice.at.bioanalytical.com) sent the message


Marco:

We've cannulated the bile duct with a shunt which allows the bile to
continue flowing into the gut without interruption, while we monitor the
chemistry of the bile via a dialysis membrane installated in the shunt.  A
quick reference to this can be found in a past issue of the journal Current
Separations (www.currentseparations.com) at:

http://www.currentseparations.com/issues/17-2/cs17-2d.pdf

The advantage of this technique is that the rat is freely-moving, and that
you conserve the bile since you are dialyzing it but not actually removing
any of it. But, the disadvantage is that with dialysis you won't recover
100% of the analyte so if you are looking for total excretion, it may not
serve your needs.  If you'd like more information, I'll send some of our
reprints on this topic.

Candice Kissinger
Physiology and In Vivo Sampling Group
BAS
West Lafayette Indiana USA

Back to the Top

Want to post a follow-up message on this topic? If this link does not work with your browser send a follow-up message to PharmPK@boomer.org with "Bile duct cannulation in rats" as the subject

PharmPK Discussion List Archive Index page