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I try to determine clavulanic acid in human plasma by HPLC. There are
some useful analytical methods described. The most interresting ones,
by derivisation. But, I could not find any hint for plasma sample
stabilization. Clavilanic acid is not stable in matrix and even
aqueous solution. Has anyone experience by a stabilization proceeding
for plasma samples? I would like to Known the adequate plasma sample
treatment. If anybody could give me a hint?
Thank you in advance.
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Further to your request regarding determination of clavulanic
acid(CLA) in human plasma by HPLC.I would like to tell you the follow:
* CLA is stable at -70 Centigrade both in aqueous and plasma, so a
stock solution of CLA is to be prepared in H2O and then diluted in
KH2PO4 buffer to give a working standard solution which should be
stored at -70 centigrade.
* CLA is plasma pending analysis should be stored at -70 centigrade.
* Plasma sample preparation:
400 micro liter + 100 microliter imidazole (pH 6.8) as
derivatizing agent should be and incubate at room temp. for 10 min.
400 microliter of this mixture was taken and then 800 microliter of
acetonitrile was added, and then centrifuged for 10 min. 1 ml of
supernatant was evaporated to dryness and the residue was
reconstituted with 100 microliter of mobile phase. 50 microliter of
the solution injected into chromatograph.
If you need more information, please let me know by e. mail.
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