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To the group,
We are assaying a compound in rat, human and dog plasma (plasma spiked with
the compound). In dog and human plasma the compound elutes as a nice single
peak. However, in rat plasma another peak appears just before the compound
peak. It seems that adding ascorbic acid reverses that to a single peak
indicating an oxidation in rat plasma. Does anybody know of any
information/reference regarding the composition differences in the plasma
of different animal species?
Thank you all for your response.
Chandrani Gunaratna
Chandrani Gunaratna, Ph.D.
Senior Research Scientist
Bioanalytical Systems
2701 Kent Avenue
West Lafayette, IN 47906
Phone: (765)463-4527
E-Mail: prema.at.bioanalytical.com
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You may wnat to look at the molecule. deduce the likelihodd of particularlly
structure(s) then use a simulation for LogP, HPLC and GC program from ACD labs.
By using this you should be able to find a chromatographic profile simular to
the one your are observing. That evidence should get you started on
confirming the structure using IR. MS or NMR.
Ed O'Connor
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Another simpler possibility exists. Instead of adding ascorbic acid, decrease
the pH with H3PO4. If you are using ion pair or ion suppression you may have
incomplete ionization of the species and.or an insufficient amount of pairn
agent. let me know what you find.
thanks
Ed o'connor
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Copyright 1995-2010 David W. A. Bourne (david@boomer.org)