PharmPK Discussion - Checking the integrity of BBB in rodents

• On 17 Oct 2013 at 09:05:04, Ravi (bravikanth.-a-.gmail.com) sent the message
  

  
  Dear All,
  
  Can anyone suggest various ways of evaluating integrity of blood brain barrier in rodent models.
  
  Warm Regards,
  
  Ravi
  
  

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• On 18 Oct 2013 at 12:06:22, Ed O'Connor (efoconnor.aaa.gmail.com) sent the message
  

  
  Evans blue
  
  

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• On 18 Oct 2013 at 13:27:21, rajneet oberoi (rajneet.oberoi.aaa.GMAIL.COM) sent the message
  

  
  Hi,
  
  You could use radioactive sucrose or inulin as a marker for BBB integrity.  You can then measure the
  concentration of either using scintillation counting (after homogenizing the brain).
  
  Alternatively, you could also use dyes such as fluorescein or texas-red which can be measured
  through fluorescence (after sectioning the brain).
  
  Best,
  
  Rajneet
  
  --
  Rajneet Oberoi, PhD Candidate
  Department of Pharmaceutics
  University of Minnesota
  Minneapolis, MN 55455
  
  

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