PharmPK Discussion - Drug extraction

• On 24 Sep 2013 at 08:54:43, Sumit Basu (sumitbasu87.at.gmail.com) sent the message
  

  
  Hi everyone,
  
  Thanks for the thread. I am also trying to extract my drug from feces and I tried some recovery
  experiments using feces as a matrix. I tried different protocols till date and could not see the
  peak of the drug even in recovery experiment. At last after heating the dry feces I got at most 20%
  recovered feces. Could you please let me know any other method I can try to enhance the recovery of
  the drug??
  
  My compound is a glycosylated compound which is water soluble. For extraction solvent I tried
  different solvents like butanol, Ethyl acetate, Methanol etc., but only in ACN worked better
  compared all the solvents.
  
  Looking for your suggestions!! Thanks!!
  
  --
  Sumit Basu
  Graduate Student & Research Assistant
  Dr. Ming Hu Lab
  Division of Pharmaceutics
  Dept. Of Pharmacological and Pharmaceutical Sciences (PPS)
  College of Pharmacy, University of Houston
  1441 Moursund Street, Room # 523
  Texas Medical Center
  Texas-77030
  USA
  
  

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• On 27 Sep 2013 at 08:29:34, sudipta basu (basu.sudipta.aaa.gmail.com) sent the message
  

  
  Hello Sumit Basu,
  
  I would like to know the nature of your analyte (Polar, No-polar, pKa etc). This will help to select
  suitable solvent and extraction techniques.
  In general:
  You need to first homogenate your feces using water or any other suitable buffer (1:3 ratio). Then
  you can try protein precipitation (PPT) using ACN like say (25 µL of homogenated fecal sample and
  add 100 µL of ACN) vortex for ~5-10 min. Centrifuge for 4000 rpm for 10 min. Take ~60 µL supernatant
  for LC-MS/MS analysis.
  
  You can also try SPE, best work for urine and fecal sample analysis.
  
  Regards,
  Sudipta Basu
  Associate Director
  DMPK and Toxicology dept.
  Sai Life Sciences Ltd, Pune, India
  
  

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