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Can anyone provide a value and a citation for normal bile flow in the
mouse
(any strain)? I've been unable to find such a value in any of the
literature citations I've pursued, or in the texts I've consulted on
hepatology, gastroenterology, or physiology. I can find lots of
information on other species, but none on mouse. Please post any
replies
to the list, or e-mail me directly. Any help will be greatly
appreciated.
E.S. Ward, Ph.D.
Absorption Systems, Inc.
stacy_ward.-a-.hotmail.com
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[A few replies - db]
From: "Odishaw, Joi L"
To: "'PharmPK.-at-.pharm.cpb.uokhsc.edu'"
Subject: RE: PharmPK Bile Flow Rate in Mice
Date: Thu, 6 Aug 1998 15:09:50 -0400
MIME-Version: 1.0
Stacy-
Pharm Research printed a commentary in 1993 that provides physiological
parameters in laboratory animals and humans. It lists the normal bile
flow in a 20g mouse as 2.0 mL/day. The original source for this data is
cited by the authors of the commentary. (B Clark and DA Smith, Crit Rev
Toxicol. 12:343-385, 1984)
Check out: Pharm Res 10 (7):1093-1095, 1993. (Authors: Brian Davies and
Tim Morris)
Hope this helps
Joi Odishaw
Glaxo Wellcome, Inc.
jlo44669.-a-.glaxowellcome.com
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Date: Fri, 7 Aug 1998 08:39:00 +0100
From: cawello.-at-.schwarzpharma.com
Sender: cawello.-at-.schwarzpharma.com
Reply-To:
Organization: Schwarz Pharma Group
To: PharmPK.-a-.pharm.cpb.uokhsc.edu
Subject: re: PharmPK Bile Flow Rate in Mice
Importance: Normal
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An interesting overview of 'physiological parameters in laboratory animals
and humans' is published in Pharmaceutical Research 1993(10): 1093-1096 by
Brain Davies and Tim Morris. The article lists a bile flow rate in mice of
2.0 mL/day. The reference to this value is Crit. Rev. Toxical 1984(12):
343-385.
Willi Cawello, PhD
Clinical Pharmacology
Schwarz Pharma, Germany
Cawello.-a-.SchwarzPharma.com
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Date: Fri, 07 Aug 1998 12:27:51 +0100
From: Hans Markus Bender
MIME-Version: 1.0
To: PharmPK.-at-.pharm.cpb.uokhsc.edu
Subject: Re: PharmPK Bile Flow Rate in Mice
X-Priority: 3 (Normal)
Hello Stacy,
a good source for your topic is:
Davies, B and Morris, T: Physiological parameters in laboratory animals
and humans, Pharmaceut. Res. 10 (7), 1093-5 ,1993
bile flow mouse (0.02kg): 2.0 mL/day
I hope that helps.
Best regards
H. M. Bender, DVM
Institute of Pharmacokinetics and Metabolism
Grafing
Merck KGaA, Germany
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X-Sender: smelethil.-at-.cctr.umkc.edu
Date: Fri, 07 Aug 1998 09:47:52 -0500
To: PharmPK.at.pharm.cpb.uokhsc.edu
From: Sri Melethil
Subject: Re: PharmPK Bile Flow Rate in Mice
Mime-Version: 1.0
In rats (250-300 gms) it (bile flow) is about 1 ml/hr. Hope this will be
of some help.
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Date: Fri, 07 Aug 1998 15:33:35
From: Janusz Byczkowski
To: PharmPK.-a-.pharm.cpb.uokhsc.edu
Subject: Re: PharmPK Bile Flow Rate in Mice
CC: stacy_ward.aaa.hotmail.com
Mime-Version: 1.0
To: stacy_ward.-at-.hotmail.com
Mouse bile flow per hour:
QBILC=0.00015 $'Bile flow (L/hr*kg-1)
transformed to total flow of bile per animal:
QBIL= QBILC*BW
For mice (and other animals animals possessing gallblader),
you have to assume that there is about 1 h
worth of bile already in the hepatobilliary
system, therefore the total volume of bile
at time T after exposure is:
VBIL = QBIL*(T+1)
where T is time [h]
I was using these as a default for mice for several years
and this default worked well for several compounds.
The problem began, when I tried to look up the reference.
Well, I do not have one (it is NOT very easy to cannulate a bile
duct in a mouse, and keep the canula unclogged for a while).
As far as I remember, I brought the very number
0.00015 L/Hr*kg-1 from a Toxicology Forum conference in
Oxford, UK, several year ago, and if I rememebr correctly,
Dr. T. Green of Imperial Chemical Industries Toxicology Lab in UK
actually was collecting 24 hr bile from B6C3F1 mice.
I hope, it will help you (at least to start the search).
Please let me know the outcome of your search.
Has anybody a better source?
Janusz Z. Byczkowski, Ph.D., D.Sc.
Senior Toxicologist
TN & Associates, Cincinnati, OH
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[Two more replies - db]
From: "Brouwer, Ken R"
To: "'PharmPK.-at-.pharm.cpb.uokhsc.edu'"
Subject: RE: PharmPK Bile Flow Rate in Mice
Date: Mon, 10 Aug 1998 08:49:53 -0400
MIME-Version: 1.0
Stacy:
In Pharmaceutical Research, Vol. 10, No. 7, 1993, pages 1093 - 1095,
Davies and Morris have a review of physiological parameters in
laboratory animals. Bile flow for a 0.02 kg mouse is 2.0 ml/day.
Original references are also provided in this article.
(Is this the Stacy from UNC?)
Regards,
Ken
Kenneth R. Brouwer, Ph.D.
US Dev BioMet
R&D 2.3075
(919) 483-5872 (w)
(919) 483-5652 (f)
(919) 260-1991 (c)
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Date: Mon, 10 Aug 1998 11:53 -0600
From: "Fiona Stavros"
To: PharmPK.-a-.pharm.cpb.uokhsc.edu
Subject: Re: PharmPK Bile Flow Rate in Mice
Dear Stacy
I see you have had a number of replies giving you information on bile
flow in mice. For general information on comparative physiological
parameters between species (surface area, hematology, gastric pH,
organ weights, respiration rates, volumes, sex diferences etc.) the
CRC Handbook of Toxicology (Ed. MJ Derelanko and MA Hollinger, CRC
Press) is a pretty good, well referenced, compendium (although it did
not have information on bile flow in the mouse, only in rat, g-pig,
rabbit, cat, dog, hen, sheep, and monkey)
Fiona Stavros, Ph.D.
Texas Biotechnology
FStavros.-at-.TBC.com
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Copyright 1995-2010 David W. A. Bourne (david@boomer.org)