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To follow the elimination of liposomal lipid, we generally use tritiated
cholesteryl hexadecyl ether as a non-metabolizable, non-exchangeable marker.
There are other markers typically used, and for this please follow the
articles published by Scherphof's group in the Netherlands. We have tried
to differentiate between released drug and liposome encapsulated drug using
non-invasive or non-perturbing imaging techniques. For this, please refer
to the Window chamber method in Diewhirst's laboratory at Duke University.
Also, do refer to the non-invasive techniques developed in Dr. Walter Wolf's
laboratory. For liposomes, tumor and tissue drug concentrations determined
represents "total" drug levels (released + encapsulated), since sample
processing methods typically employed tend to disrupt the liposome bilayer
causing leakage of drug. Therefore, for meaningful interpretations of data,
it is useful to differentiate between intact and non-intact carriers, as you
Trust this is of some help.
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