PharmPK Discussion - Nicotine-Cotinine assay

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On 29 Jul 1998 at 11:12:28, "Iavarone, Laura Else" (li14270.-at-.GlaxoWellcome.co.uk) sent the message


Does anyone of you  know  a very sensitive method for the assay of very
low concentrations of nicotine and its metabolite cotinine in  rat
plasma?
I would be interested also in getting in contact with Contract Houses.

I am dealing with 50-100=B5L of plasma samples and concentrations
estimated to be below the 60ng/mL.
I would like to see at least 10ng/mL for both compounds.

Any assistance would be helpful.

	Laura Iavarone

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On 30 Jul 1998 at 13:36:36, "Richard Sams PhD." (sams.3.-at-.osu.edu) sent the message


Dear Dr. Iavarone:

Several years ago the University of Kentucky Tobacco Research Institute
funded the development of ELISA assays to measure cotinine and nicotine in
rat and mice serum samples. The cotinine-ELISA was the better of the two
assays. It had an I-50 of approximately 1.0 ng/ml in serum. This assay also
cross reacted with the nicotine metabolite trans-3'-hydroxycotinine. The
nicotine-ELISA was not as sensitive with an I-50 closer to 100 ng/ml in
serum. The nicotine-ELISA showed little cross-reactivity with related
compounds. Both assays required only 20 uL of sample but could be modified
to use less sample, if necessary.

I do not know whether these assays were ever marketed. Elisa Technologies
(Division of Neogen Corporation in Lexington, Kentucky) may have had an
interest in marketing kits for the determination of these substances. They
can be reached at 1-800-477-8201.

Richard A. Sams, PhD
Professor and Laboratory Director
Analytical Toxicology Laboratory
College of Veterinary Medicine
The Ohio State University
Telephone: 614-292-5730
Fax: 614-292-4299
email address: sams.3.at.osu.edu

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On 4 Aug 1998 at 13:54:59, "Zutshi, Anup" (zutshi.-a-.BATTELLE.ORG) sent the message


Dear Dr. Iavarone:

We have developed and validated an assay for substituted nicotine and
cotinine in rat, dog and human plasma using LC-MS/MS. Using a 0.5 mL plasma
sample we can quantify down to 2 ng/mL. We are confident we can reproduce
this assay for nicotine and cotinine. Please give me a call if you need any
further information.

Anup Zutshi
zutshi.-a-.battelle.org
(614) 424-5997 (Phone)
(614) 424-3268 (Fax)

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