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Dear colleagues,
I am looking for information on non-heparin anticoagualation techniques to
allow for repeated blood sampling in rats. Are there for example problems
with the use of citrate? Your reply is most apprecitated.
Regards,
Mendel Jansen
Leiden/Amsterdam Center for Drug Research
Division of Pharmaceutical Technology
P.O. Box 9502, 2300 RA Leiden
The Netherlands
Phone: +31 71 527 4219
Fax: +31 71 527 4565
Email: M.Jansen.at.LACDR.LeidenUniv.nl
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Hi,
According to my experinces with multiple blood sampling in rats, you can
use saline itself the cannula remains open if you flush it with saline.
Regards
Majid
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[A few replies - db]
X-Sender: pmayo.aaa.pop.srv.ualberta.ca
Date: Wed, 08 Jul 1998 16:20:45
To: PharmPK.-a-.pharm.cpb.uokhsc.edu
From: Patrick Mayo
Subject: Re: PharmPK Re: Non-heparin anticoagulation
Mime-Version: 1.0
Greetings!
Most hospitals in Canada use bacteriostatic saline in a heparin lock on a
cannula and it works fine. I have found in my work on rats heparin is
sufficient, but may also depend on the study. I was working with L-NAME
which by decrease nitric
oxide levels leads to an increase in platelet aggregation, without 100 U/mL
heparin in the cannula, I had great difficulty with coagulation.
Therefore, if the drug you are studying has any pro-coagulant effects I
would opt for some heparin.
- Pat
---
From:
Date: Wed, 8 Jul 1998 20:47:41 EDT
To: PharmPK.-a-.pharm.cpb.uokhsc.edu
Mime-Version: 1.0
Subject: Re: PharmPK Re: Non-heparin anticoagulation
a meta-analysis of human subjetcs in hospitals stated that saline alone was =
as
good as heparin to mainatinn the patency of hep-locks. This analysis was
performed in the early 90's, but I'm sorry I don't have the citation.
However based upon this analysis many hospitals switched from SASH (saline-
antibiotic-salne-heparin) to SAS (saline-antibiotic-saline) administration.
HRR, RPh
---
X-Sender: zambon064.-a-.dialinsrv.dialin.unisource.it
Date: Thu, 09 Jul 1998 11:16:15 +0200
To: PharmPK.-a-.pharm.cpb.uokhsc.edu
From: Fabio MACCHI
Subject: Re: PharmPK Non-heparin anticoagulation
Mime-Version: 1.0
Have you tried with EDTA?
Another possibility is a solution of 200 mg/ml of Ascorbic Acid in 0.5M
EDTAK3 (commercilly available) add 10=B5l of this solution for each ml of
blood collected
Regards
Fabio Macchi
Dr. Fabio Macchi
Pharmacokinetics Lab.-ZambonGroup S.p.A.
fabio.macchi.aaa.zambongroup.com
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[Two replies - db]
Date: Thu, 09 Jul 1998 10:54:08 -0600
From: Steven J. Weber
To: PharmPK.-a-.pharm.cpb.uokhsc.edu
Subject: PharmPK Re: Non-heparin anticoagulation -Reply
Mime-Version: 1.0
DO NOT use EDTA in the cannula! EDTA is a chelating agent.
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Reply-To: "David H. Ellis" <.aaa.erinet.com>
From: "David H. Ellis"
To:
Subject: Heparinized vs. nonheparinized Saline
Date: Fri, 10 Jul 1998 06:23:59 -0400
MIME-Version: 1.0
X-Priority: 3
We have found that in rats that clots begin to form after a few hours if
heparin is not in the line (clots begin when the sampling interval becomes 2
hours); however, in dogs, we use saline only and have no problems.
Dave
David H. Ellis
Procter & Gamble Pharmaceuticals
dhellis.-a-.erinet.com
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